Anti-CD31 (PECAM1) [JC70] recombinant antibody
Invented at University of Oxford
- Datasheet
- References (13)
- Inventor Info
Info
| Catalogue Number | 154812 |
| Applications | N/A |
| Antigen/Gene or Protein Targets | PECAM1 (CD31) |
| Synonyms | Platelet/Endothelial Cell Adhesion Molecule 1; CD31 Antigen; EndoCAM; PECAM-1; GPIIA; PECA1; CD31/EndoCAM; CD31 |
| Reactivity | Human |
| Relevance |
Recombinant monoclonal antibody directed against endothelial cell marker (CD31, JC70), capable of evaluating tumour angiogenesis and angiosarcoma more effectively than anti-FVIII-Reg. Background and Research Application CD31/JC70, also known as platelet endothelium cell adhesion molecule (PECAM-1), is a 130kDa transmembrane glycoprotein found on cell surface of endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, lymphocytes (T cells, B cells, and NK cells), megakaryocytes, and osteoclasts. PECAM1 is a member of the immunoglobulin (Ig) superfamily. It is also a major constituent of the endothelial cell intercellular junction and expressed in certain cancers. PECAM1 is involved in cell signalling and adhesion, trans-endothelial migration of leukocytes, angiogenesis, and integrin activation. PECAM1 is a specific marker of endothelial differentiation. This is a recombinant version of the anti-CD31 monoclonal antibody . Anti-CD31 be used to identify the presence of endothelial cells in a histological tissue sample to determine the degree of angiogenesis (which in turn can determine tumour severity/growth). Anti-CD31 stains a fixation resistant epitope on endothelial cells in both benign and malignant endothelial cells in angiosarcomas with more consistency than monoclonal or polyclonal antibodies to FV111-Rag . C70 is a good marker of endothelial cells in paraffin sections. Anti-CD31 was created to recognise membrane bound glycoprotein CD31/JC70. |
| Host | Mouse |
| Immunogen | Cell membrane preparation from the spleen of a patient with hairy cell leukaemia |
| Subclass | IgG1 |
| Research Area | Adhesion, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Stem Cell Biology |
| Immunogen UniProt ID | P16284 |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Anti-CD31 is useful in studying benign and malignant human vascular disorders in processed tissue samples. It reacts with endothelial cells in a wide range of tissues, with specific reactions to endothelium when staining fixed tissue. Anti-CD31 allows for analysis of cutaneous angiosarcomas. Anti-CD31 is more evenly distributed and reliable than commonly used marker factor VIII antibody, used as a vascular endothelial cell marker. Immunocytochemistry (ICC) 1:10-1:100 Immunofluorescence (IF) 1:10-1:100 Immunohistochemistry (Paraffin) (IHC (P)) 1:100 Flow Cytometry (FACS) 1:2 Western Blot (WB) 1:1-1:100 Concentration 1mg/ml as standard |
References: 13 entries
A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway.
Ding et al. 2013. PLoS One. 8(5):e63628. PMID: 23675495.
HIF-1α transgenic bone marrow cells can promote tissue repair in cases of corticosteroid-induced osteonecrosis of the femoral head in rabbits.
JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections.
Muller WA, Kishimoto T, et al (eds) 1997. Leucocyte Typing VI, Garland Publishing Inc., New York and London, p 362-364. ISBN-13: 978-0815327455
Novel modeling of cancer cell signaling pathways enables systematic drug repositioning for distinct breast cancer metastases.
Orecchia et al. 2011. PLoS One. 6(9):e24307. PMID: 21931678.
Parums et al. 1990. J Clin Pathol. 43(9):752-7. PMID: 2212067.
Sirtinol treatment reduces inflammation in human dermal microvascular endothelial cells.
Tissue factor-bearing microparticles and CA19.9: two players in pancreatic cancer-associated thrombosis?
Tsuneki et al. 2015. Lab Invest. :. PMID: 25961170.
Woei-A-Jin et al. 2016. Br J Cancer. :. PMID: 27404454.
Zhao et al. 2013. Cancer Res. 73(20):6149-63. PMID: 24097821.
Add a reference
References: 13 entries
A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway.
Ding et al. 2013. PLoS One. 8(5):e63628. PMID: 23675495.
HIF-1α transgenic bone marrow cells can promote tissue repair in cases of corticosteroid-induced osteonecrosis of the femoral head in rabbits.
JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections.
Muller WA, Kishimoto T, et al (eds) 1997. Leucocyte Typing VI, Garland Publishing Inc., New York and London, p 362-364. ISBN-13: 978-0815327455
Novel modeling of cancer cell signaling pathways enables systematic drug repositioning for distinct breast cancer metastases.
Orecchia et al. 2011. PLoS One. 6(9):e24307. PMID: 21931678.
Parums et al. 1990. J Clin Pathol. 43(9):752-7. PMID: 2212067.
Sirtinol treatment reduces inflammation in human dermal microvascular endothelial cells.
Tissue factor-bearing microparticles and CA19.9: two players in pancreatic cancer-associated thrombosis?
Tsuneki et al. 2015. Lab Invest. :. PMID: 25961170.
Woei-A-Jin et al. 2016. Br J Cancer. :. PMID: 27404454.
Zhao et al. 2013. Cancer Res. 73(20):6149-63. PMID: 24097821.
Add a reference
