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Recombinant Antibodies

Anti-Integrin b3 [Y2/51] recombinant antibody

Invented at University of Oxford
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Catalogue Number 154827
Applications N/A
Antigen/Gene or Protein Targets Integrin ß3 (CD61; GPIIIa)
Synonyms Integrin Subunit Beta; Integrin; Beta 3 (Platelet Glycoprotein IIIa; Antigen CD61); Platelet Membrane Glycoprotein IIIa; Antigen CD61; GPIIIa; GP3A; Platelet Glycoprotein IIIa; Integrin Beta 3; CD61 Antigen; BDPLT16; BDPLT2; CD61; GT
Reactivity Human
Relevance Recombinant antibody which can detect CD61 marker on megakaryocytes.

Background and Research Application
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells and aberrant integrin expression has been found in many epithelial tumours.
CD61/ integrin b3 is a glycoprotein found on megakaryocytes, osteoclasts, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. This is a recombinant version of anti-integrin B3 monoclonal antibody, which is widely used as a marker of megakaryocytes in bone marrow trephines.
Host Mouse
Immunogen Phytohaemagglutin-stimulated peripheral blood cells
Subclass IgG1
Research Area Adhesion, Cardiovascular, Cell Signaling & Signal Transduction
Immunogen UniProt ID P05106
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

Western Blot (WB) 1:100-1:500

Immunohistochemistry (Paraffin)

(IHC (P)) 1:50-1:100

Flow Cytometry (FACS) 2 ug/test

Concentration
1mg/ml as standard

References

There are 9 reference entries for this reagent.

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References: 9 entries

Brune et al. 2007. Eur Cell Mater. 14:78-90

Complex formation of platelet membrane glycoproteins IIb and IIIa with fibrinogen.

discussion 90-1. PMID: 18085506.

Gatter et al. 1988. Histopathology. 13(3):257-67. PMID: 3192191.

Immunological study of in vitro maturation of human megakaryocytes.

In vitro comparison of human fibroblasts from intact and ruptured ACL for use in tissue engineering.

Nachman et al. 1982. J Clin Invest. 69(2):263-9. PMID: 6460044.

The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens.

Vinci et al. 1984. Br J Haematol. 56(4):589-605. PMID: 6231944.


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References: 9 entries

Brune et al. 2007. Eur Cell Mater. 14:78-90

Complex formation of platelet membrane glycoproteins IIb and IIIa with fibrinogen.

discussion 90-1. PMID: 18085506.

Gatter et al. 1988. Histopathology. 13(3):257-67. PMID: 3192191.

Immunological study of in vitro maturation of human megakaryocytes.

In vitro comparison of human fibroblasts from intact and ruptured ACL for use in tissue engineering.

Nachman et al. 1982. J Clin Invest. 69(2):263-9. PMID: 6460044.

The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens.

Vinci et al. 1984. Br J Haematol. 56(4):589-605. PMID: 6231944.


Add a reference

Inventor Information

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Invented at

University of Oxford
University of Oxford

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