NHE-3 expressing PS120 cell line
Invented at Johns Hopkins University
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Info
| Catalogue Number | 156414 |
| Antigen/Gene or Protein Targets | Na+/H+ exchanger |
| Parental Line | CCL39 |
| Disease Keywords | Diarrhea and constipation |
| Relevance |
An isoform of the Na+/H+ exchanger, NHE-3, has been cloned, sequenced and stably expressed in PS120 fibroblast cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNA was cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then stably transfected using double selection with G418 and acid selection. The Na+/H+ exchange activity of these cells were proven using fluorescence measurement using a pH sensitive dye. These cells are ideal for the Na+/H+ exchanger functional studies as well as drug effect studies with null background. Additional advantages include: • NHE-3 is essential in studies related to diarrhea and constipation |
| Research Area | Cell Signaling & Signal Transduction, Drug Discovery & Development |
