- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 157701 |
| Antigen/Gene or Protein Targets | ADAM10 |
| Parental Line | HEK 293T |
| Synonyms | A Disintegrin And Metalloproteinase domain-containing protein 10, CRISPR-Cas9 |
| Host | Human |
| Tissue | Kidney |
| Disease Keywords | Cancer, Alzheimer’s and inflammatory diseases |
| Relevance | A disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer’s and inflammatory diseases. ADAM10 is a ‘molecular scissor’ that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines. |
| Research Area | Cancer, Neurobiology, Other |
| Notes |
CRISPR edited ADAM 10 HEK-293T cells. Generation of CRISPR/Cas9-knockout cell line: A guide RNA sequences was selected for human ADAM10, and the following primer pairs were used to encode these sequences: ADAM10 guide 2 (5’-CACCGATACCTCTCATATTTACAC-3’ and 5’-AAACGTGTAAATATGAGAGGTATC-3’). Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license. |
References: 2 entries
Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex
Brummer et al. 2018. FASEB J. 32(7):3560-3573. PMID: 29430990.
Add a reference
References: 2 entries
Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex
Brummer et al. 2018. FASEB J. 32(7):3560-3573. PMID: 29430990.
Add a reference
Inventor Information
Inventors
|
Michael Tomlinson |
