Retinal pigmented epithelial cell line overexpressing HA-FLAG-CXCR4-MYC
Invented by Dr. Allen Liu
Invented at University Of Michigan
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 157704 |
| Antigen/Gene or Protein Targets | CXCR4 |
| Parental Line | Retinal pigmented epithelial cell line |
| Host | Human |
| Tissue | Eye |
| Relevance | Retinal pigment epithelial cells (RPE) stably expressed human CXCR4 when transduced with N-terminal FLAG or N-terminal FLAG and C-terminal MYC tagged CXCR4. RPE cells were used because this cell line has very low CXCR4 expression. Results can be attributed to the overexpression of CXCR4 and not be confounded by endogenous CXCR4. As an experimental model the cells showed overexpressed CXCR4 recapitulate endogenous CXCR4 signalling and internalization dynamics. |
| Production Details | Stable RPE cell lines expressing HA-FLAG-CXCR4 or HA-FLAG-CXCR4-MYC receptors were generated using lentiviral transduction produced from the pLVX vector |
| Research Area | Cell Signaling & Signal Transduction |
References: 1 entry
References: 1 entry
Inventor Information
Inventors
|
Dr. Allen Liu |
