Bovine Alveolar Type II (BATII) cell line
Invented by Diane Lee at University of Surrey
Bovine Type II Alveolar Epithelial (B2AE) cell line
Invented by Diane Lee
Invented at University of Surrey
- Datasheet
- References (3)
- Inventor Info
Info
| Catalogue Number | 157737 |
| Tissue | Alveolus |
| Model | Immortalised Line |
| Relevance | Differentiates into Alveolar Type I (ATI)-like cells when cultured at air-liquid interface, generating a mixed population of Alveolar Type II (ATII) and ATI in a pseudostratified epithelial cell layer. Used in co-culture with bovine pulmonary arterial endothelial cells as a model of the bovine alveolus. |
| Production Details | Generated by the lentiviral transduction of Bmi1 and hTERT into primary bovine alveolar type II epithelial cells at passage 1. Proliferation rate as for primary. |
| Research Area | Immunology, Other |
| Growth/Phenotype Keywords | Cobblestone. Fixed (2D), 3D (matrigel) or air-liquid interface (ALI) |
| Recommended Growing Conditions | Small airway growth medium (Lonza) or Small airway epithelial cell medium (Promocell). 95 % air/5 % CO2, 37oC humidified incubator. Subculture routine: 0.25 % trypsin/1 mM EDTA 5 minutes at 37oC. Neutralise with medium/10 % FBS. Spin, count and subculture ΒΌ dilution to minimum of 3 x 10,000/cm2. Yield at subculture: ~5 x 10,000/cm2. |
References: 3 entries
Lee et al. 2019. Curr Protoc Toxicol. 80(1):e71. PMID: 30875462.
Lee et al. 2019. F1000Res. 8:357. PMID: 31448101.
Lee et al. 2018. Sci Rep. 8(1):11927. PMID: 30093682.
Add a reference
References: 3 entries
Lee et al. 2019. Curr Protoc Toxicol. 80(1):e71. PMID: 30875462.
Lee et al. 2019. F1000Res. 8:357. PMID: 31448101.
Lee et al. 2018. Sci Rep. 8(1):11927. PMID: 30093682.
Add a reference
