Anti-Xenopus NK cells [1G5] monoclonal antibody
Invented by John Horton at University of Durham
Catalogue Number | 157743 |
Applications | FACS IHC |
Antigen/Gene or Protein Targets | Xenopus Natural Killer (NK) cells |
Reactivity | Xenopus laevis |
Relevance | Enables identification of specific lymphoid populations in Xenopus (tested in liver, spleen and gut), namely NK cells. Enables separation from T and B cells. |
Host | Mouse |
Immunogen | Mice were immunised with splenocytes from early-thymectomized (Tx) Xenopus following B cell and thrombocyte depletion, therefore an enriched Natural Killer (NK) cell population. |
Immunogen UniProt ID | Not applicable |
Positive Control | IgG2a kappa |
Molecular Weight (kDa) | 72 |
Myeloma Used | P3X63Ag8.653 |
Recommended Growing Conditions | RPMI 1640 + 10% FCS |
Notes | Behaves relatively poorly in western blotting, however does identify bands similar to antibody clone 1G5. |
Research Area | Cancer, Cell Type or Organelle Marker, Immunology |