HeLa FRT/TO (YFP-Astrin Δ70 siRNA res) cell line
Invented by Duccio Conti , Viji M. Draviam
Invented at Queen Mary University of London
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 157865 |
| Parental Line | HeLa cell line |
| Synonyms | HeLa FRT/TO YFP-Astrin WT cell line conditionally expressing siRNA-resistant YFP-Astrin WT. |
| Host | Human |
| Tissue | Cervix |
| Model | Cancer Model |
| Production Details |
HeLa FRT/TO YFP-Astrin Δ70 cell line conditionally expressing siRNA-resistant YFP-Astrin Δ70. Created by transfection of HeLa Flp recombinase cell line with pCDNA5-FRT/TO-YFP-Astrin Δ70 expression plasmid, followed by a brief Hygromycin selection and sorting for YFP positive using FACS. |
| Research Area | Cell Type or Organelle Marker, Cell Structure and Motility, Cell Signaling & Signal Transduction, Cell Cycle, Cancer |
| Notes |
siRNA oligos to target Astrin mRNA 5’ UTR (GACUUGGUCUGAGACGUGAtt) or Astrin 52 oligo (UCCCGACAACUCACAGAGAAAUU). Astrain Δ70 mutant was generated by PCR amplification of 1–1122 coding region of Astrin and subcloning into the desired expression vector. |
References: 1 entry
References: 1 entry
Inventor Information
Inventors
|
Duccio Conti |
|
|
Viji M. Draviam |
