Anti-PKD (calreticulin) polyclonal antibody
Invented by Abdo Alnabulsi
Invented at Vertebrate Antibodies Limited
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Info
| Catalogue Number | 158061 |
| Applications | ELISA WB |
| Antigen/Gene or Protein Targets | Tetracapsuloides bryosalmonae parasite antigen (calreticulin) |
| Reactivity | Tetracapsuloides bryosalmonae |
| Relevance |
Proliferative kidney disease (PKD), caused by the myxozoan parasite Tetracapsuloides bryosalmonae, is one of the most economically damaging diseases to trout aquaculture. T. bryosalmonae has a two-host life cycle, as other myxosporeans, cycling between freshwater bryozoan and salmonid fish species. It is characterised by a swollen kidney and spleen, bloody ascites, and pale gills, indicating the fish becomes anemic at the late stage of the disease. This parasite is allowed to cross the renal tubules wall to proliferate within the interstitial tissue of kidney, inducing a chronic lymphoid hyperplasia marked by a strong parasite-driven immunosuppressant pathogenesis and a dysregulation of T-helper subsets. Fish recovering from PKD develop protective immunity to subsequent parasite challenge, providing the impetus for vaccine development. Polyclonal antibody used to identify T. bryosalmonae antigens will be able to elicit protective immune responses for developing DNA vaccines. Calreticulin released by an intracellular parasite is capable of entering the cytoplasm, which is able to be processed via MHC class I. It is could compete with host calreticulin for the binding of MHC molecule, and thus interfere with peptide loading and presentation. This is a polyclonal antibody used for the diagnosis of T. bryosalmonae parasite. It is a valuable tool to monitor Calreticulin role in parasite development as well as immune modulation of the host. |
| Host | Rabbit |
| Immunogen | Ovalbumin-conjugated synthetic peptide. |
| Formulation | Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal |
| Molecular Weight (kDa) | 41 |
| Notes |
Applications and Recommended Usage Conditions: - ELISA recommended to be used at 1:500 dilution. Peptide immunogen or recombinant protein as positive control. - WB recommended to be used at 1:500 dilution. Recombinant protein as positive control. Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal |
| Positive Control | ELISA: peptide immunogen and recombinant protein WB: recombinant protein |
| Research Area | Cell Type or Organelle Marker, Immunology |
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Inventor Information
Inventors
|
Abdo Alnabulsi |
