- Datasheet
- References (0)
- Inventor Info
Info
| Catalogue Number | 158062 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Tetracapsuloides bryosalmonae parasite antigen |
| Reactivity | Tetracapsuloides bryosalmonae |
| Relevance |
Proliferative kidney disease (PKD), caused by the myxozoan parasite Tetracapsuloides bryosalmonae, is one of the most economically damaging diseases to trout aquaculture. T. bryosalmonae has a two-host life cycle, as other myxosporeans, cycling between freshwater bryozoan and salmonid fish species. It is characterised by a swollen kidney and spleen, bloody ascites, and pale gills, indicating the fish becomes anemic at the late stage of the disease. This parasite is allowed to cross the renal tubules wall to proliferate within the interstitial tissue of kidney, inducing a chronic lymphoid hyperplasia marked by a strong parasite-driven immunosuppressant pathogenesis and a dysregulation of T-helper subsets. Fish recovering from PKD develop protective immunity to subsequent parasite challenge, providing the impetus for vaccine development. A polyclonal antibody used to identify T. bryosalmonae antigens will be able to elicit protective immune responses for developing DNA vaccines. This is a useful diagnostic tool to identify T. bryosalmonae parasite. This antibody is potentially neutralizing providing a valuable research tool to study parasite-host interactions and vaccine development. |
| Host | Rabbit |
| Immunogen | Ovalbumin-conjugated synthetic peptide. |
| Formulation | Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal |
| Molecular Weight (kDa) | 31 |
| Notes |
Applications and Recommended Usage Conditions: - ELISA recommended to be used at 1/500 dilution. Peptide immunogen as positive control. - WB recommended to be used at 1/500 dilution. Recombinant protein, parasite infected head- kidney tissue lysate. - IHC recommended to be used at 1/1000 dilution. Recommended Usage Conditions: ELISA neat; Western Blot neat; IHC neat (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0). Unpurified anti-serum from rabbit preserved in 0.02% Thiomersal |
| Positive Control | ELISA: peptide immunogen, recombinant protein WB: recombinant protein IHC: formalin-fixed, paraffin-embedded parasite infected head-kidney tissues |
| Research Area | Cell Type or Organelle Marker, Immunology |
References
There are 0 reference entries for this reagent.
References: 0 entry
There is no reference for this reagent yet, feel free to use the button below to suggest one.
Add a reference
References: 0 entry
There is no reference for this reagent yet, feel free to use the button below to suggest one.
Add a reference
Inventor Information
Inventors
|
Abdo Alnabulsi |
