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Vectors

αCat-Vinc-HA vector

Invented by Dr Claudio Franco from Instituto de Medicina Molecular João Lobo Antunes
Invented at Instituto de Medicina Molecular João Lobo Antunes
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(A) Diagram showing the molecular structure of the αCat-Vinc construct which this vector encodes. αCat-Vinc-HA is a fusion protein containing the β-catenin-binding domain of α-catenin (brown) fused with the actin-binding domain of vinculin (gray) and the HA tag (blue). (B) Example of HUVECs expressing αCat-Vinc-HA. Nucleus labeled with Dapi, adherens junctions with VE-Cadherin. Scale bar, 20 µm. Blue square 1 (bottom left) shows a higher magnification of a reticular junction where HA does not co-localise with VE-Cadherin. Blue square 2 (bottom right) shows a higher magnification of a linear junction where HA and VE-cadherin co-localise. (PMID: 31246175)
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Catalogue Number 158343
Selectable Markers Chloramphenicol and Ampicillin
Vector Type pLX303
Research Area Adhesion
Notes Lentiviral plasmid pLX303 encoding the following fusion protein: the β-catenin-binding domain of α-catenin fused with the actin-binding domain of vinculin and the HA tag.

References

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References: 1 entry

Carvalho et al. 2019. Elife. 8:. PMID: 31246175.

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References: 1 entry

Carvalho et al. 2019. Elife. 8:. PMID: 31246175.

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