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Immorta-MAIT T cell clone EM-C5 cell line

Invented at Oregon Health & Science University

Info

Catalogue Number 159688
Host Human
Disease Keywords Mycobacterium tuberculosis
Model Immortalised Line
Relevance Mucosal-associated invarient T (MAIT) cells are innate-like T cells (a subset of T cells) that are found in blood, liver, lungs, and mucosa and are known to play a role in defense against bacterial and viral infections. MAITs have also been shown to potentially play a role in autoimmune diseases such as multiple sclerosis, rheumatiod arthritis, and systemic lupis erythematosus.
Production Details Generated by single cell isolation of MR1/5-OP-RU tetramer+ cells from a human thymus. Single cells were rapidly expanded into a T cell clone using antibody to CD3 (clone OKT3) and IL-2.
Conditional No
Research Area Immunology, Bacteriology
Growth/Phenotype Keywords TRAV1-2 is expressed uniformly (determined by flow cytometry staining). This clone expresses CD4, CD8, CD161, and CD27 and can be classified as a double-positive thymocyte. This T cell clone binds the MR1/5-OP-RU tetramer but not MR1/6FP tetramer (negative control). Clone is MR1-restricted in its production of IFN-gamma by ELISPOT test, determined by its response to M. smegmatis-infected A549 cell line but not a M. smegmatis-infected MR1-\- A549 cell line.
Recommended Growing Conditions Can be use with conventional T cell expansion methods and proliferates under simple culture methods. Cell line can be maintained for at least 3 months.

Cultures can be established by centrifugation with subsequent resuspension at 1 x 10^5 viable cells/mL in complete RPMI-1640 medium (10% heat-inavtivated FBS). Optional recombinant IL-2 at 1ng/mL.

T-25 flask is recommended for culturing. Recommended concentration to maintain cultures between 1x10^5 and 1x10^6 viable cells/mL. Fresh medium recommended weekly, based on density and growth rate.

References

There are 3 reference entries for this reagent.

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References: 3 entries

Laugel et al. 2016. J Immunol. 197(3):971-82. PMID: 27307560.

Corbett et al. 2014. Nature. 509(7500):361-5. PMID: 24695216.

Gold et al. 2010. PLoS Biol. 8(6):e1000407. PMID: 20613858.


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References: 3 entries

Laugel et al. 2016. J Immunol. 197(3):971-82. PMID: 27307560.

Corbett et al. 2014. Nature. 509(7500):361-5. PMID: 24695216.

Gold et al. 2010. PLoS Biol. 8(6):e1000407. PMID: 20613858.


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