Anti-CRF polyclonal antibody (Whole Serum, Sheep)
Invented at Flinders University
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Info
| Catalogue Number | 160415 |
| Applications | ELISA IHC IF |
| Antigen/Gene or Protein Targets | An 18 amino acid synthetic CRF peptide (H-CEQLAQQAHSNRKLMEII-OH), conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Reactivity | Human, Mouse, Rat and Sheep |
| Host | Sheep |
| Notes |
FORM: Whole Serum, Lyophilised SPECIFICITY: Specific for CRF. Confirmed by Immunohistochemical analysis of Paraffin Embedded Rat Brain (Hypothalamus) as well as Rat and Mouse whole-mount Ileum tissue. IMMUNO-HISTOCHEMISTRY: Use at 1:100 dilution for Immunofluorescence staining and 1:4000 – 1:6000 dilution for DAB Staining on Paraffin Embedded Sections. Use at 1:1000 – 1:4000 dilution on whole-mount tissue. The optimal dilution should be determined by the end user. STORAGE: Long term - 70ºC dry Short term - 20ºC solution or 4ºC with appropriate antibacterial agent. Avoid repeated freezing and thawing. Glycerol (1:1) can be added for additional stability. PRODUCT PREPARATION: Reconstitute with addition of 50 µl of sterile water. KNOWN USES: ELISA (1:100 to 1:10,000), Immunohistochemistry (1:1000 – 1:6000), Immunofluorescence Staining (1:100). |
| Research Area | Fluorescent Cell Imaging, Other, Structural Biology |
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