Anti-GFAP polyclonal antibody (Whole Serum, Sheep)
Invented at Flinders University
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Info
| Catalogue Number | 160417 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | A 20 amino acid synthetic GFAP (Glial Fibrillary Acidic Protein) peptide (H-CKLADVYQAELRELRLRLDQ-OH), conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Reactivity | Human, Rat and Sheep |
| Notes |
FORM: Whole Serum, Lyophilised SPECIFICITY: Specific for GFAP. Confirmed by Immunohistochemical and Western Blot analysis of Paraffin Embedded Human and Rat Brain. IMMUNO-HISTOCHEMISTRY: Use at 1:500 – 1:1000 dilution for Immunofluorescence staining, 1:4000 – 1:8000 dilution for DAB Staining on Paraffin Embedded Sections and 1:5000 -1:10,000 for Western Blotting. The optimal dilution should be determined by the end user. STORAGE: Long term - 70ºC dry Short term - 20ºC solution or 4ºC with appropriate antibacterial agent. Avoid repeated freezing and thawing. Glycerol (1:1) can be added for additional stability. PRODUCT PREPARATION: Reconstitute with addition of 100 µl of sterile water. KNOWN USES: ELISA (1:100 to 1:10,000), Immunohistochemistry (1:4000 – 1:8000), Immunofluorescence Staining (1:500 – 1:1000), Western Blot (1:5000 – 1:10,000) |
| Research Area | Fluorescent Cell Imaging, Other, Structural Biology |
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