Anti-SQSTM1 polyclonal antibody (Affinity Purified, Rabbit)
Invented at Flinders University
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Info
| Catalogue Number | 160428 |
| Applications | WB |
| Antigen/Gene or Protein Targets | Synthetic peptide as part of human Ubiquitin Interactive Motif (UIM) derived from p62 protein, also known as SQSTM1 conjugated to Keyhole Limpet Hemocyanin (KLH). (accession: AAH01874) |
| Reactivity | Human and Rabbit |
| Host | Rabbit |
| Method of Purification | Affinity Purified |
| Notes |
FORM: Affinity Purified, Lyophilised SPECIFICITY: Confirmed by Immunohistochemical/Western Blot analysis of normal and diseased human brain. IMMUNO-HISTOCHEMISTRY: Use at 1-4 ug/ml. The optimal dilution should be determined by the end user. This antiserum works superbly in both frozen and paraffin embedded tissues. STORAGE: Long term - 70ºC dry Short term - 20ºC solution or 4ºC with appropriate antibacterial agent. Avoid repeated freezing and thawing. Glycerol (1:1) can be added for additional stability. PRODUCT PREPARATION: Reconstitute with addition of 20 µl of sterile water. KNOWN USES: Western Blot: The antibody recognizes 4 major bands of 130, 62, 45 and 22 kDa in human brain homogenate. The antibody may also recognize some proteins containing SQSTM1 domains from other species as 80, 62 and 47 kDa proteins are identified in rat sympathetic ganglia. Pre-incubation of the antibody with the immunizing peptide abolishes all reactivity in both human brain homogenate and rat sympathetic ganglia. |
| Research Area | Other, Structural Biology |
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