CHO DC-SIGN WT cell line

Invented by Alessandra Cambi

Invented at Radboud UMC

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AFM-assisted cell-cell force spectroscopy to measure the interaction strength of single CHO-DC-SIGN cells and C. albicans. (A) Parental CHO cells and cells expressing DC-SIGN-WT were incubated with FITC-labeled C. albicans in the presence or absence of blocking agents; anti-DC-SIGN antibody and EDTA. The percentage of cells that bound C. albicans was calculated by flow cytometry. Data are presented as means ± S.D. (B) Schematic set-up showing a single C. albicans cell immobilized on a tip-less AFM cantilever interacting with a CHO-DC-SIGN cell. (C) Example brightfield image that shows how a C. albicans cell on the tip (indicating by dashed white ellipse) is positioned to interact with four different CHO-DC-SIGN cells. The positions at which the C. albicans is brought into contact are indicated by asterisks. (D) Example FD-curves of C. albicans interacting with parental CHO, CHO-DC-SIGN-WT with addition of 30 µg/ml anti-DC-SIGN blocking antibody for 30 minutes.Taken from Te Riet J, Joosten B, Reinieren-Beeren I, Figdor CG, Cambi A. N-glycan mediated adhesion strengthening during pathogen-receptor binding revealed by cell-cell force spectroscopy. Sci Rep. 2017;7(1):6713. Published 2017 Jul 27. doi:10.1038/s41598-017-07220-w

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Catalogue Number	160716
Antigen/Gene or Protein Targets	Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin
Parental Line	CHO
Synonyms	DC-SIGN
Host	Hamster
Tissue	Ovary
Disease Keywords	HIV-1, Ebola Virus, Hepatitis C virus
Relevance	Dendritic Cell-Specific ICAM-3-Grabbing Non-Integrin (DC-SIGN; CD209) is a type II plasma membrane PRR abundantly expressed in antigen-presenting cells such as dendritic cells (DCs) and activated macrophages. As member of the C-type Lectin Receptor (CLR) family, DC-SIGN binds a plethora of pathogens, ranging from viruses like HIV-1, ebola virus, and hepatitis C virus, to larger pathogens like Mycobacterium tuberculosis and Candida albicans.
Production Details	CHO cell lines stably expressing DC-SIGN wild-type were established by Lipofectamin 2000 transfection, and were cultured in Ham’s F-12 medium supplemented with 10% heat-inactivated FBS, 1% Antibiotic Antimycotic Solution , and 0.5 mg/ml of the aminoglycoside antibiotic G418.
Conditional	No
Research Area	Cell Signaling & Signal Transduction, Immunology
Recommended Growing Conditions	Ham’s F-12 medium supplemented with 10% heat-inactivated FBS, 1% Antibiotic Antimycotic Solution , and 0.5 mg/ml of the aminoglycoside antibiotic G418.

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CHO DC-SIGN-N80A cell line

Invented by Alessandra Cambi at Radboud UMC

Antigen/Gene or Protein Targets: Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin lacking the ligand binding domain N80.

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