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Venus-A2AR vector

Invented by Luísa V Lopes
Invented at Instituto de Medicina Molecular João Lobo Antunes
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Adapted from Temido-Ferreira et al., 2020. Mol Psychiatry; 25(8): 1876–1900. Figure 1. Increased levels of A2AR impair calcium homeostasis. a Control immunocytochemistry analysis of neurons transfected with Venus-A2AR construct confirmed co-expression of Venus and A2AR. b Representative images of Ca2+ imaging. Bright regions indicate the location of cytoplasm and organelles, where the concentration of Ca2+ is higher than in the dark regions indicating the extracellular medium, where diffusion processes take place. The right image corresponds to the ratio between the radiation emitted at 510 nm, when cells are excited at 340 nm, over emission upon excitation at 380 nm (F340/F380).
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Catalogue Number 160728
Bacterial Resistance Amp
Selectable Markers Neomycin
Vector Type pcDNA3.1( + )
Synonyms A2AR
Antigen/Gene or Protein Targets Adenosine A2A receptor
Relevance A construct encoding a Venus-A2AR fusion protein to explore the synaptic function of A2AR
Research Area Fluorescent Cell Imaging, Neurobiology
Notes Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5'-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3' and 5'-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3', and the pair of primers 5'-GCCACCATGGGCAGCAGC-3' and 5'-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3'. PCR fragments were cloned into the vector pcDNA3.1( + ) linearized with HindIII and XbaI, and the resulting construct was verified by DNA sequencing (GATC Biotech, Germany).

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References: 1 entry

Temido-Ferreira et al. 2020. Mol Psychiatry. 25(8):1876-1900. PMID: 29950682.

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References: 1 entry

Temido-Ferreira et al. 2020. Mol Psychiatry. 25(8):1876-1900. PMID: 29950682.

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