Tnao38 subclone cell line
Invented at Boyce Thompson Institute
- Datasheet
- References (3)
- Inventor Info
Info
| Catalogue Number | 160776 |
| Parental Line | High Five (T. ni) |
| Synonyms | BTI-Tnao38 |
| Relevance |
The insect cell based baculovirus expression system is a well established and widely used system for the production of recombinant proteins A Trichoplusia ni derived insect cell line shown to be highly productive, in both quality and quantity, in the expression of recombinant proteins via the baculovirus expression system. Tnao38 cells have been shown to be exceed protein expression of both Sf9 and High Five cell lines (Hashimoto et al.,2010). |
| Production Details | Thawing Procedure: Remove cells from liquid nitrogen and thaw quickly in a 37°C water bath. Centrifuge gently (10º C, 800 rpm, 5 min) to pellet cells. Resuspend cell pellet in pre-warmed (27°C) medium and transfer to T25 flask. Grow at 27°C. |
| Conditional Description | Tnao38 is a HighFive (Trichoplusia ni) sub-clone accidentally isolated from a primary culture of Ascalapha odarata cells |
| Research Area | Gene Expression, Other |
| Growth/Phenotype Keywords | Morphology: Flat fibroblast-like appearance at lower densities and more spherical appearance at higher cell densities and in suspension. Doubling time: 20.2 hrs in adherent cell culture ( see Hashimoto et al. 2010 ). Passage when ~90% confluent (Dislodge) |
| Recommended Growing Conditions | TNMFH+10%FBS [1] in T25, T75, or T150 flasks at 27°C |
| Notes |
Absence of alphanodavirus based on RT-PCR (Hashimoto et al., 2010) Link to original HighFive clone confirmed by SNP |
| Cellosaurus ID | CVCL_Z252 |
References: 3 entries
Wilde et al. 2014. Biotechnol Lett. 36(4):743-9. PMID: 24375231.
Hashimoto et al. 2012. BMC Biotechnol. 12:12. PMID: 22531032.
Hashimoto et al. 2010. BMC Biotechnol. 10:50. PMID: 20602790.
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References: 3 entries
Wilde et al. 2014. Biotechnol Lett. 36(4):743-9. PMID: 24375231.
Hashimoto et al. 2012. BMC Biotechnol. 12:12. PMID: 22531032.
Hashimoto et al. 2010. BMC Biotechnol. 10:50. PMID: 20602790.
Add a reference
