4-trifluoromethyl-7-hydrazinyl-2H-chromen-2-one (TFCH) biomolecular fluorophore small molecule (tool compound)
Invented by Susan Bane , Kamalika Mukherjee
Invented at Binghamton University
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 160832 |
| Synonyms | TFCH |
| Type | Fluorescent Probe |
| Relevance |
4-trifluoromethyl-7-hydrazinyl-2H-chromen-2-one (TFCH) is a novel fluorescent biocompatible probe has been developed to detect oxidative damage in live cells and tissues in as little as 5 minutes. TO accomplish this, TFCH utilizes the irreversible reaction of radicals with biomolecules that result in the covalent addition of carbonyls to biomolecules. This probe has been shown to outperform other commercially available toxicity and reactive oxygen species assays. This probe has been shown to be highly sensitive. The limit of detection is orders of magnitude smaller than other previously reported fluorophores resulting in less reagent needed to detect carbonylation. TFCH is rapid, live cell and tissue compatible and also be used in fixed cells and tissues. |
| Key Attributes |
Detection of Oxidative Stress damage in a myriad of cells and tissues. Detection of an early biomarker for disease, toxicity, cell and tissue damage. Use in live cells and tissues, fixed cells and tissues and existing biopsy material. |
| In vivo applications | Renal tissue imaging |
| In vitro applications | Live cell screening |
| Research Area | Drug Discovery & Development, Fluorescent Cell Imaging |
References: 2 entries
A Highly Sensitive Fluorogenic Assay for the Detection of Nephrotoxin-Induced Oxidative Stress in Live Cells and Renal Tissue
Mukherjee et al. 2021. ACS Sens. 6(7):2523-2528. PMID: 34214393.
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References: 2 entries
A Highly Sensitive Fluorogenic Assay for the Detection of Nephrotoxin-Induced Oxidative Stress in Live Cells and Renal Tissue
Mukherjee et al. 2021. ACS Sens. 6(7):2523-2528. PMID: 34214393.
Add a reference
Inventor Information
Inventors
|
Susan Bane |
|
|
Kamalika Mukherjee |
