HeLa Flp-In SLC7A5
Invented by Matthias Hentze at European Molecular Biology Laboratory
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- References (1)
- Inventor Info
Info
| Catalogue Number | 160841 |
| Antigen/Gene or Protein Targets | YBX3 |
| Parental Line | HeLa FLP-IN T-REx |
| Model | Knock-Out |
| Relevance | To identify genes whose expression is controlled by YBX3 |
| Production Details | Adherent HeLa cells were grown at 37°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO) |
| Research Area | Metabolism |
| Recommended Growing Conditions | Grown in medium containing blasticidine (5mg/ml) and hygromycin (200mg/ml). |
| Notes | Cells were grown until 70%–80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturer’s recommendations (Lipofectamine RNAiMax, Invitrogen, cat# 137780755). YBX3 and control siRNAs were obtained from Dharmacon (YBX3 pool: SMARTpool ON-Targetplus L-015793-00 or YBX3 3′ UTR targeting: ON-Targetplus L-015793-07; and control pool: ON-Targetplus non-targeting siRNAs 1-4). The siRNA used to target the YBX3s 3′ UTR was YBX3 smartpool J-015793-07. Cells were harvested 48 hours after transfections. |
HeLa Flp-In YBX3: GFP
Invented by Matthias Hentze at European Molecular Biology Laboratory
HeLa Flp-In SLC7A5+SLC7A5 3′ UTR
Invented by Matthias Hentze at European Molecular Biology Laboratory
HeLa Flp-In SLC25A6+SLC7A5 3′ UTR
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References: 1 entry
The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance
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References: 1 entry
The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance
Add a reference
Inventor Information
Inventors
|
Matthias Hentze |
