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Catalogue Number
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160855 |
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Antigen/Gene or Protein Targets
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Antigen, protein fragment, receptor binding domain
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Parental Line
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HEK293F
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Synonyms
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Recombinant SARS-CoV-2 RBD
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Host
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Human
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Disease Keywords
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Coronavirus, COVID-19, SARS-CoV-2
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Relevance
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The SAR-CoV-2 RBD fragment expressed by this cell line has been successfully used to make ELISA assays for detection of coronavirus infection in human patients.
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Production Details
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To constitutively express SARS-CoV-2 RBD, HEK293F cell line was genetically modified in the lab using the lentivirus system. More specifically, the SARS-CoV-2 RBD gene was inserted into the genome of HEK293F cells using lentiviruses, and the stably transfected cells constitutively secrete SARS-CoV-2 RBD into the cell medium at high levels. The RBD fragment has 8X his tags for subsequent purification.
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Research Area
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Drug Discovery & Development, Immunology, Structural Biology, Virology
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Recommended Growing Conditions
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The cells are cultured in FreeStyle 293 Expression Medium from Thermo Fisher.
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Positive Control
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No
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