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Monoclonal Antibodies

IL-17F (MM17F8.F5)

Invented by Van Snick Jacques, Donckers Dominque, Bar Isabelle and Uyttenhove Catherine
Invented at Ludwig Institute for Cancer Research
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Anti-GM-CSF, anti-IL-17F, and anti-IL-17E/IL-25 auto-vaccination. (A) Sera from control and GM-CSF–OVAglu-immunized C57BL/6 mice tested by ELISA on biotinylated mouse GM-CSF or control cytokine (human IL-9) bound to neutravidin-coated plates and by inhibition of FDC-P1 cell proliferation induced by GM-CSF (●, no antiserum) or IL-3 (■, no antiserum). (B) Anti-IL-17F antibodies in sera of C57BL/6 mice vaccinated with IL-17F–OVAglu on IL-17F-coated ELISA plates (IL-17A as control) and inhibition by MM17F-8F5, a mAb derived from these mice, of IL-17F-induced neutrophil chemotactic activity produced by IL-17A−/− splenocytes activated under Th17 conditions and tested on IL-17A−/− splenic leukocytes as responders. Bar graph shows mean chemotactic index (±sem) for each group compiled from six measurements. (C) Anti-IL-17E/IL-25 antibody detection by ELISA in sera from C57BL/6 mice vaccinated with IL-17E/IL-25–OVAglu and by inhibition of IL-5 induction by IL-17E/IL-25 in bone marrow cells from RAG−/− BALB/c mice incubated with 10 ng/ml IL-17E/IL-25. **P<0.01 (Student's t test).
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Catalogue Number 160877
Applications ELISA
Antigen/Gene or Protein Targets IL-17F (Interleukin 17F, CTLA-8)) is a cytokine belonging to the IL-17 family
Reactivity N/A
Host Mouse
Positive Control The positive control is the IL-6 produced in response to IL-17F without antibody.
Recommended Growing Conditions 3T3 based IL-17A/F bioassay 3T3 seeding Celles are seeded in 48w plates, 30 000 cells/w in 250ul of DMEM 10% FCS Cells are incubated 24h 37° 5% CO2
Notes Treatment of the cells
Discard the culture medium and replace by 100 ul medium containing
-30ng /ml murine IL-17A
-100 ng murine IL-17F
If you want to test inhibition activity of mAbs, préincubate a mixture of IL-17 and serial dilutions of the mAbs ( from 1000ng/ml to ± 1 ng/ml) in separated wells (min 30 min, usually around 4H) before adding to the 3T3 cells.
Incubate the cells + mAbs + IL17 min 24H, preferably up to 40H. Collect SN from each well and store frozen till IL-6 Elisa.
IL-6 ELISA – dilute SN 1/10 to perform the murine IL-6 ELISA.
PS. Some FCS batches induce rapidly a shift in the 3T3 cells that become either insensitive to IL-17 or stop IL-6 secretion in response. Prepare quite a large batch of frozen vials as soon as possible.
Research Area Immunology

References

There are 1 reference entries for this reagent.

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References: 1 entry

Uyttenhove et al. 2011. J Leukoc Biol. 89(6):1001-7. PMID: 21385949.

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References: 1 entry

Uyttenhove et al. 2011. J Leukoc Biol. 89(6):1001-7. PMID: 21385949.

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Inventor Information

Inventors

Van Snick Jacques, Donckers Dominque, Bar Isabelle and Uyttenhove Catherine

Van Snick Jacques, Donckers Dominque, Bar Isabelle and Uyttenhove Catherine

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Invented at

Ludwig Institute for Cancer Research
Ludwig Institute for Cancer Research

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