MEF RAF1 KO Cell Line
Invented at University Of Leicester
Raf KO Mouse
Invented at University Of Leicester
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151476 |
| Antigen/Gene or Protein Targets | Raf1+/- heterozygous knockout |
| Disease Keywords | Cancer; Apoptosis |
| Relevance | The Raf-/- embryo phenotype shows defects in vascularisation and placental development as well as increased apoptosis of many tissues. |
| Production Details | Raf-1 targeting vectors, where exons 10-13 were replaced with a resistance marker, were transfected into 129Ola ES cells. Successfully targeted ES cells containing homologous recombination events were selected and injected into blastocysts, Chimeric offspring were mated to MF-1 outbred background and the the C57BL6 inbred strain. |
| Conditional | No |
| Growth/Phenotype Keywords | Vascularization, Placental Development, Apoptosis |
| Mouse Genetic Background/Cross History | 129Ola/MF-1 or 129Ola/C57BL6 |
| Zygosity | Heterozygous. Homozygous Raf-1-/- mice die in embryogenesis. |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Genetic Studies Tools, Neurobiology |
References: 2 entries
Hüser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
MEK kinase activity is not necessary for Raf-1 function.
Europe PMC ID: 11296227
Add a reference
References: 2 entries
Hüser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
MEK kinase activity is not necessary for Raf-1 function.
Add a reference
