Raf KO Mouse
Invented at University Of Leicester
MEF RAF1 KO Cell Line
Invented at University Of Leicester
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151576 |
| Antigen/Gene or Protein Targets | RAF1 |
| Host | Mouse |
| Tissue | Embryo |
| Disease Keywords | Cancer |
| Model | Knock-Out |
| Relevance | The MEF RAF1 KO Cell Line allows in vivo study of Raf-1 knockout and Ras signalling and can be used to carry out drug screening toxicology studies. MEF are produced from the Raf-1+/- knockout mouse. |
| Production Details | Embryos were dissected and cut, washed several times with cold PBS and then incubated at 4°C for 2 hours in 0.25% trypsin. Cells were incubated as per the growth conditions. Primary cells were immortalised and resulting cells grown out by continuous culture. |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Drug Discovery & Development, Metabolism |
| Recommended Growing Conditions | DMEM, 10% FCS, 100U/mol penicillin/streptomycin |
| Notes | See the Raf-1 knockout mouse in related products. |
| Cellosaurus ID | CVCL_6D89 |
References: 2 entries
Hüser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
MEK kinase activity is not necessary for Raf-1 function.
Europe PMC ID: 11296227
Add a reference
References: 2 entries
Hüser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
MEK kinase activity is not necessary for Raf-1 function.
Add a reference
