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Recombinant Antibodies

Anti-MUC1, Recombinant [HMFG2]

Invented by Prof Joy Burchell from King's College London , Prof Joyce Taylor-Papadimitriou from King's College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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ELISA showing equivalence in performance between anti-MUC1, Recombinant [HMFG2] ("recombinant HMFG2") and Anti-MUC1 [HMFG2] ("purified HMFG2"). ELISA was performed on a 60mer peptide which consists of 3 tandem repeats of MUC1. The secondary anti-mouse antibody was HRP linked and the binding identified with a coloured substrate, O-phenylenediamine. Data kindly provided by Dr. Joy Burchell and Prof. Joyce Taylor-Papadimitriou
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Catalogue Number 153187
Applications ELISA FACS IHC IP WB
Antigen/Gene or Protein Targets Mucin1 (MUC1)
Synonyms ADMCKD, ADMCKD1, Breast carcinoma associated antigen DF3, Breast carcinoma-associated antigen DF3, CA 15-3, CA15 3, CA15 3 antigen, CA15.3, Cancer antigen 15-3, Carcinoma associated mucin, Carcinoma-associated mucin, CD 227, CD227
Reactivity Human
Relevance Recombinant antibody which detects several glycoforms of MUC1, a marker of breast cancer.

Background and Research Application
Mucin-1 (MUC1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MUC1 is also localised on the surface of the human milk fat globule. MUC1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development and can be used to monitor response to breast cancer treatment and disease recurrence. Lower levels over time may be indicative of a positive response to treatment. It is upregulated in the lactating breast and in carcinomas. MUC1 also is involved in tumour progression and transcription through regulation of p53.
This is a recombinant version of the anti-MUC1 antibody. Anti-MUC1 can react with unglycosylated MUC1, and several glycoforms.
Host Mouse
Immunogen Milk fat globule followed epithelial cells cultured from milk
Subclass IgG1
Formulation PBS
Concentration 1mgml-1
Research Area Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction
Immunogen UniProt ID P15941
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
This antibody immunostains human breast cancer tissues.

Clone also called 3.14.A3 or F314AE11.

The product of the MUC1 gene is the polymorphic epithelial mucin. SM3 reacts with a core protein epitope defined as PDTRP.
Recombinant monoclonal antibody produced from the original monoclonal. Manufactured using Absolute Antibody’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma.

*** PLEASE NOTE: Applications stated are those corresponding to original hybridoma **

3 different variants available:
- Anti-MUC1 [HMFG2], Mouse IgG1, Lambda
- Anti-MUC1 [HMFG2], Mouse IgG1, Lambda, engineered Fc domain containing key point mutations that abrogate binding to Fc gamma receptors
- Ab00712-23.0 Anti-MUC1 [HMFG2]

Concentration
1mg/ml as standard

References

There are 2 reference entries for this reagent.

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References: 2 entries

Original hybridoma first published in: Taylor-Papadimitriou et al. 1981. Int J Cancer. 28(1):17-21. PMID: 7309278.

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Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture.

Europe PMC ID: 7309278

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References: 2 entries

Original hybridoma first published in: Taylor-Papadimitriou et al. 1981. Int J Cancer. 28(1):17-21. PMID: 7309278.

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Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture.

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Inventor Information