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Cell Lines

PROP Flp reporter cell line

Invented by Sergio F de Almeida
Invented at Instituto de Medicina Molecular João Lobo Antunes
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New reporter genes allow visualisation of transcription upon DNA damage with single-molecule resolution. (A) Illustration of a HEK293 cell containing a single FRT genomic locus for single copy integration of the reporter gene. (B) Schematic of the two reporter genes with the I–SceI site and MS2/PP7 sequences highlighted. (C and D) Representative images of PROP (C) and EX2 (D) reporter cells. Images were acquired at the indicated time points after doxycycline treatment. Examples of pre-mRNAs diffusing throughout the nucleoplasm are numbered 1 to 4. The transcription site (TS) of the reporter gene is indicated in each cell. Corresponding images depicting the diffraction limited objects in the highest intensity plane are shown below. (Sci Adv. 2019 Jan 9;5(1):eaau1249. doi: 10.1126/sciadv.aau1249. eCollection 2019 Jan)
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Catalogue Number 158330
Parental Line Flp-In™ T-REx™ 293 cell line
Host Human
Tissue Kidney
Model Reporter
Production Details The pOG44 Flp-recombinase expression vector was co-transfected with the PROmoter-Proximal (PROP) reporter construct into Flp-In™ T-REx™ 293 cells
Conditional No
Research Area DNA Damage and Repair, Fluorescent Cell Imaging, Genetic Studies Tools
Recommended Growing Conditions maintain under selection with 200 µg/ml Hygromycin B and 15 µg/ml Blasticidin at 37°C in a humidified atmosphere with 5% CO2.

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Vítor et al. 2019. Sci Adv. 5(1):eaau1249. PMID: 30662944.

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References: 1 entry

Vítor et al. 2019. Sci Adv. 5(1):eaau1249. PMID: 30662944.

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Inventor Information