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EX2 Flp reporter cell line

Invented by Sergio F de Almeida
Invented at Instituto de Medicina Molecular João Lobo Antunes
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New reporter genes allow visualisation of transcription upon DNA damage with single-molecule resolution. (A) Illustration of a HEK293 cell containing a single FRT genomic locus for single copy integration of the reporter gene. (B) Schematic of the two reporter genes with the I–SceI site and MS2/PP7 sequences highlighted. (C and D) Representative images of PROP (C) and EX2 (D) reporter cells. Images were acquired at the indicated time points after doxycycline treatment. Examples of pre-mRNAs diffusing throughout the nucleoplasm are numbered 1 to 4. The transcription site (TS) of the reporter gene is indicated in each cell. Corresponding images depicting the diffraction limited objects in the highest intensity plane are shown below. (Original publication: Sci Adv. 2019 Jan 9;5(1):eaau1249. doi: 10.1126/sciadv.aau1249. eCollection 2019 Jan)
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Catalogue Number 158338
Parental Line Flp-In™ T-REx™ 293 cell line
Host Human
Tissue Kidney
Model Reporter
Production Details The pOG44 Flp-recombinase expression vector was co-transfected with the EX2 reporter construct into Flp-In™ T-REx™ 293 cells
Conditional No
Research Area DNA Damage and Repair, Fluorescent Cell Imaging, Genetic Studies Tools
Recommended Growing Conditions Maintain under selection with 200 µg/ml Hygromycin B (Roche) and 15 µg/ml Blasticidin (InvivoGen) at 37°C in a humidified atmosphere with 5% CO2.
Notes Derived from embryonic kidney cells

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References: 1 entry

Vítor et al. 2019. Sci Adv. 5(1):eaau1249. PMID: 30662944.

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References: 1 entry

Vítor et al. 2019. Sci Adv. 5(1):eaau1249. PMID: 30662944.

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Inventor Information